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X-linked inhibitor of apoptosis protein (XIAP) is a member of the inhibitor of apoptosis protein (IAP) family that includes cIAP1. XIAP is a negative regulator of various apoptotic stimuli and death pathways and is the most potent caspase inhibitor of the IAP family. XIAP contains BIR domains, like other IAP family proteins, which interact with the IAP-binding motif of caspases. This interaction is implicated in the control of pro-apoptotic and anti-apoptotic activities. XIAP compound characterization on the BIR2 and BIR3 domains enables accurate profiling and selectivity studies.
XIAP is an attractive target in cancer therapies since it’s overexpression in tumor cells is a well-described mediator of resistance to chemotherapy and it has been linked to tumor aggressiveness. Several therapeutic strategies targeting XIAP have been investigated, such as SMAC mimetics. Additionally, XIAP displays E3 ligase activity and leads to targeted proteins' ubiquitination and degradation, allowing this property to be exploited via a Proteolysis-targeting chimera (PROTAC) strategy. Therefore, new compounds targeting XIAP provide dual roles, the inhibition of XIAP anti-apoptotic function and the induction of targeted protein degradation.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Points (200-50,000)||500 Assay Points|
|Assay Target||XIAP BIR3|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been ...
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.