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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Thymic stromal lymphopoietin (TSLP) is a cytokine that plays a critical role in the maturation of T cells through antigen presenting cells. TSLP is mainly produced by epithelial cells and its expression is regulated by many other cytokine such as IL1β and TNFα. TSLP expression is linked to many disease states such as asthma, inflammatory arthritis, atopic dermatitis, eczema, eosinophilic esophagitis and other allergic states. Thus, TSLP becomes one of many important biomarkers for airway inflammatory diseases. This kit is designated to detect and quantify the levels of TSLP in cell culture media and other biological matrices.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
This manual explains how to run the AlphaLISA no-wash human TSLP detection assay.