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AlphaLISA TruHits Biotin-free Detection Kit, 1,000 Assay Points

Bead control set for identifying false positives and interfering compounds.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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1,000 Assay Points
2133.00 USD
10,000 Assay Points
14200.00 USD
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The AlphaLISA TruHits kit is designed as a tool for AlphaLISA users to identify false positives in AlphaLISA HTS assays early in the screening process, when working with biotin-rich media. This kit includes AlphaLISA Digoxigenin TruHits Acceptor and Anti-Digoxigenin Fab Fragment Donor beads which interact together to generate an AlphaLISA signal. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. The AlphaLISA Biotin-Free TruHits kit allows the identification of light scattering molecules (insoluble compounds), singlet oxygen quenchers and Digoxigenin mimetics interfering with the AlphaLISA signal, and thus facilitates the detection of false positives. However, TruHits kits do not allow systematic identification of all interfering compounds.


Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Format Microplates
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 1,000 Assay Points