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The AlphaLISA TruHits kit is designed as a tool for AlphaLISA users to identify false positives in AlphaLISA HTS assays early in the screening process, when working with biotin-rich media. This kit includes AlphaLISA Digoxigenin TruHits Acceptor and Anti-Digoxigenin Fab Fragment Donor beads which interact together to generate an AlphaLISA signal. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. The AlphaLISA Biotin-Free TruHits kit allows the identification of light scattering molecules (insoluble compounds), singlet oxygen quenchers and Digoxigenin mimetics interfering with the AlphaLISA signal, and thus facilitates the detection of false positives. However, TruHits kits do not allow systematic identification of all interfering compounds.
|Experimental Type||In vitro|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.