PerkinElmer
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TREM2 (human) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® immunoassay kit for human TREM2 enables the quantitative determination of human TREM2 in buffer, cell culture media, serum, plasma, cerebrospinal fluid and cell supernatant using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

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Unit Size
List Price
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AL3109HV
100 Assay Points
782.00 USD
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AL3109C
500 Assay Points
2213.00 USD
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AL3109F
5,000 Assay Points
11100.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

TREM2 is a transmembrane molecule expressed on myeloid cells. It acts as the receptor for an unknown ligand to activate myeloid cells such as dendritic cells, increasing phagocytic activity. Recently, TREM2 has been shown to be involved in neurodegenerative diseases such as ataxia, early dementia and Alzeimer’s disease. Elevated levels of TREM2 have been noted in cerebrospinal fluid as a response to Alzeimer’s disease.

Specifications

Assay Target TREM2
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 100 Assay Points
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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

Manual

Protocol for AlphaLISA human TREM2 assay

This manual describes how to run the AlphaLISA human TREM2 assay kit.

PDF 661 KB