The AlphaLISA® human TNFα/TNFR1 binding kit is designed for the detection of binding activity between human TNFα and TNFR1, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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The AlphaLISA® human TNFα/TNFR1 binding assay uses anti-6xHis AlphaLISA Acceptor beads to capture the His-tagged TNFR1 and Streptavidin-coated donor beads to capture the biotinylated TNFα. Donor beads and Acceptor beads come into proximity through TNFα binding to TNFR1. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Tumor necrosis factor alpha (TNFα) is an immunity-modulating cytokine that is required for defense against infectious diseases and carcinogenesis. TNFα activates signals through its two receptors [TNF receptor 1 (TNFRI) and TNF receptor 2 (TNFRII)]. TNFR1 is ubiquitously expressed and can be fully activated by both the membrane-bound and soluble trimeric forms of TNFα, whereas TNFR2 is found mostly on certain populations of immune cells and respond to the membrane-bound form of the TNFα homotrimer. However, a block of TNFα-mediated host defense often increases the risk of bacterial or viral infection or of the development of lymphoma. Thus, a thorough understanding of the function of the TNFα-TNFR binding is important for the design of optimal therapies against the various TNFα-related autoimmune diseases.
|Assay Target Class||Cytokine, Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
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