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TIM-3 (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Unit Size
List Price
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AL3052HV
100 Assay Points
842.00 USD
 
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AL3052C
500 Assay Points
2526.00 USD
 
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AL3052F
5,000 Assay Points
20200.00 USD
 
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

TIM-3 (T-cell immunoglobulin and mucin-domain containing-3), also known as HAVCR2 (Hepatitis A virus cellular receptor 2), is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. TIM-3, as an immune checkpoint marker, belongs the famility of inhibitory receptors such as PD-1 and LAG3. TIM-3 is up‑regulated on several populations of activated myeloid cells and T cells (Th1, CD8+, NK, Treg) and plays critical roles in immunosuppressive functions and in anti-tumor immunity. Its binding to Galectin-9 induces a range of immunosuppressive activities which enhance immune tolerance and inhibit anti-tumor immunity. For example, TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. This kit is designated to detect and quantify the levels of TIM-3 in cell culture supernatant, serum, and cell lysates.

Specifications

Assay Target TIM-3
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Oncology
Unit Size 500 Assay Points
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Application Note

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In this application note, you will learn:

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Brochure

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Manual

AlphaLISA human TIM-3 Detection Kit

This manual describes how to run an AlphaLISA human TIM-3 detection assay.

PDF 597 KB