The AlphaLISA® Human Microtubule-associated Protein Tau (TAU) Detection Kit is designed for detection and quantitation of human TAU in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies used in the kit correspond to clone numbers BT2 and Tau 12.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Microtubule-associated Protein Tau (TAU) is a neuronal protein that binds to axonal microtubules and has roles in the assembly of microtubules, cytoskeletal structure, and axonal transport. Six isoforms of Tau have been described, ranging from 352 to 441 residues, all originating from the alternative splicing of one gene designated MAPT. Tau interacts with tubulin to stabilize the microtubules, but its phosphorylation blocks this association, resulting in disruption of microtubule organization. In humans, hyperphosphorylated Tau can self-assemble into very insoluble aggregates (paired helical filaments) leading to various pathologies. Tauopathy is the name given to diseases associated with Tau accumulation, among which Alzheimer’s disease (AD) is one of the best known. Tau is a biochemical marker for this neurodegenerative disease as an increase of Tau in cerebrospinal fluid is observed in most patients with AD.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.