Somatostatin (human) AlphaLISA Detection Kit, 100 Assay Points

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

In an AlphaLISA competition assay, a Biotinylated analog of the analyte of interest, the tracer, binds to the Streptavidin-coated Alpha Donor beads, while the Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of low analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. In the presence of high analyte, the beads are separated resulting in lower emission.

Somatostatin is a short peptide hormone (14 or 28 amino acids) involved in regulating the endocrine system. This kit detects both active forms of peptide (Somatostatin-14 and Somatostatin-28). It plays a role in neurotransmission and cell proliferation. Somatostatin interacts with the G protein-coupled Somatostatin receptor to inhibit insulin and glucagon secretion. This peptide exists in almost all vertebrates; coded by six different genes (SS1-6). Classified as an inhibitory hormone, Somatostatin inhibits the release of growth hormone and thyroid-stimulating hormone in the pituitary and multiple gastrointestinal hormones: gastrin, CCK, secretin, and GIP.