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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
S100 calcium-binding protein A4 (S100A4) is a protein encoded by the S100A4 gene and is a member of the S100 family of proteins. In the cell, S100 proteins are localized mainly to the cytoplasm and the nucleus and regulate cellular processes. S100A4 has been well characterized for its role in cancer, particularly, enhanced cell growth and mobility leading to tumor progression and metastasis. This protein has been regarded a valuable biomarker since its expression level has been correlated with metastasis potential of many types of cancer, specifically, lung, prostate, breast and bone cancers. The S100A4 AlphaLISA kit was designed to detect S100A4 in serum, plasma, CSF, cell culture supernatants and cell lysates.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This manual explains how to run the AlphaLISA no-wash human S100A4 detection assay.