The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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For research use only. Not for use in diagnostic procedures.
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AlphaLISA technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA competition assay, a Biotinylated analog of the analyte of interest, the tracer, binds to the Streptavidin-coated Alpha Donor beads, while the Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of low analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm (Figure 2). In the presence of high analyte, the beads are separated resulting in lower emission.
Progesterone is a steroid hormone that plays a role in the menstrual cycle and pregnancy. Progesterone directs pregnancy in a multitude of ways through changes in carbohydrate, protein, and lipid metabolism. Most progesterone is produced in the corpus luteum. This hormone plays an important role in the nervous system as a neurosteroid, where it serves as a precursor to allopregnanolone. Progesterone serves as a metabolic intermediate for many of the corticosteroids and sex hormones.
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|