PerkinElmer
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PD-1/PD-L1 (mouse) AlphaLISA Binding Kit, 5,000 Assay Points

The AlphaLISA® mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL580C
500 Assay Points
2123.00 USD
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AL580F
5,000 Assay Points
10600.00 USD
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Detail Information

Basic immunoregulation studies using human cells can be challenging, whereas options for mouse cells are extensive (immunization of donor mice to generate T cells, T cell receptor transgenic mice). It can often be quicker to learn about immunology and regulation of immune responses by using a mouse system. However, working in mouse systems can often require developing separate mouse reagents if your human drug or antibody won't cross-react with mouse. The AlphaLISA mouse PD-1/PD-L1 binding kit allows determination of cross-reactivity of human anti-PD-1 or PD-L1 reagents with mouse proteins, as well as development or screening of novel mouse PD-1 and PD-L1 reagents that block the binding of this interaction. Using the AlphaLISA mouse PD-1/PD-L1 binding assay, small molecule antagonists or large molecule therapeutics and blocking antibodies can be screened in microplate format. Please note an AlphaLISA human PD-1/PD-L1 binding assay is also available for concurrent studies.

AlphaLISA Binding Assay Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad range of affinities
  • High avidity
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of binding interactions in a highly sensitive, quantitative, reproducible and user-friendly mode. In the AlphaLISA assay, a biotinylated mouse PD-L1 binds to the Streptavidin-coated Alpha Donor beads, while His-tagged mouse PD-1 is captured by Anti-6X-His AlphaLISA Acceptor beads. When PD-1 binding to PD-L1 occurs, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target PD-1, PD-L1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Mouse
Therapeutic Area Cancer
Unit Size 5,000 Assay Points