PerkinElmer
check quantity

Pan Mouse IgG Fc AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for detection and quantitation of pan mouse IgG Fc in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fc region of any mouse IgG.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL584HV
100 Assay Points
1510.00 USD
 
more
AL584C
500 Assay Points
1846.00 USD
 
more
AL584F
5,000 Assay Points
12200.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well, using a 100 μL reaction volume (10 μL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In mice, the IgG class is further divided into isotypes such as IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The molecular structure of IgG is well known as a Y-like shape that contains Fab and Fc regions. The mouse IgG Fc portion is widely used in genetic humanization technology and production of human antibodies in transgenic animals. There are more than a dozen Fc-fusion protein drugs are available on the market to treat various human diseases. The Pan Mouse IgG Fc Specific AlphaLISA Detection Kit specifically detects the Fc regions of mouse IgG subtypes (IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target Pan Mouse IgG Fc
Assay Target Class Antibody
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA®
Shipping Condition Blue Ice
Unit Size 5,000 Assay Points