PerkinElmer
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p62 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for p62 enables the quantitative determination of p62 in human cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

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Unit Size
List Price
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AL3097HV
100 Assay Points
750.00 USD
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AL3097C
500 Assay Points
2067.00 USD
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AL3097F
5,000 Assay Points
10600.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

The ubiquitin binding protein p62, or Sequestosome 1 (SQSTM1), is expressed in most cell types. Synonyms are p62 lck ligand, zeta interacting protein (ZIP, rat), A170 (mouse), OSIL, or PDB3. The human protein is 440 amino acids long. It contains an N-terminal protein binding domain (PB1) that interacts with different protein kinases and allows homopolymerization of p62. The domain is followed by several other protein-protein interaction domains and nuclear localization and export signals. Notably, the C-terminal ubiquitin association domain recognizes both mono and polyubiquitin and the LIR motif allows direct binding to the autophagosome-associated protein LC3. p62 plays an important role in the formation of intracellular protein aggregates, autophagy, and NF-κB signaling. Mutation of p62 is associated with Paget’s disease (abnormal bone turnover).

Specifications

Assay Target p62
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Autophagy
Unit Size 100 Assay Points
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Manual

Protocol for AlphaLISA human p62 Detection Kit

Protocol for AlphaLISA human p62 Detection Kit

PDF 545 KB