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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a 76-amino acid sequence cleaved from the N-terminal end of brain natriuretic peptide. NT-proBNP is an inactive protein that is used for the screening for acute congestive heart failure. Serum and plasma levels of NT-proBNP varies between individuals and generally increase with age. The physiological role of NT-proBNP is not clear, while the function of BNP is to protect the cardiovascular system from volume overload by regulating salt and water homeostasis. NT-proBNP has a longer biological half-life and therefore is the preferred biomarker for detection and analysis.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
Data and protocol for AlphaLISA NT-proBNP detection kit