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IL-17A (mouse), lyophilized (AlphaLISA), 0.1 μg

Mouse Interleukin-17 (IL-17A) standard, lyophilized, for use in running standard curves in AlphaLISA® no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat IL-17A kit but can be ordered separately.

For research use only. Not for use in diagnostic procedures.

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AL588S
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Overview

This standard should be reconstituted in 100 μL Milli-Q® grade water. The reconstituted analyte should be used within 60 minutes or aliquoted into screw-capped polypropylene vials and stored at -20 °C for further experiments. Avoid multiple freeze-thaw cycles. One vial contains an amount of analyte sufficient for performing 10 standard curves.

Specifications

Assay Target IL-17A
Assay Target Class Cytokine
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation, Auto-Immunity
Unit Size 0.1 μg
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Application Note

Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

Alpha technology offers a simple, straight forward workflow. No wash needed!

  1. Add sample
  2. Add Acceptor bead mix; incubate 1 hr
  3. Add Donor beads; incubate 30 mins
  4. Read on Alpha-enable microplate reader

Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

PDF 1 MB

Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

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Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liquid handling, assays and reagents, imaging and detection systems, and informatics.

Working independently or together, with our solutions you can achieve consistent and accurate results. By accelerating the identification and characterization of effective and safe drug candidates, the PerkinElmer portfolio enables you to optimize efficiency in your lab and deliver more actionable, real-world results.

Download the brochure to learn more about how we can partner with you so that you can discover smarter, more effective, data-driven breakthroughs in the critical screening stages of drug discovery and development.

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