MMP-2 (human) AlphaLISA Detection Kit, 5,000 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Matrix Metalloproteinase 2 (MMP2) or type IV collagenase contains 551 amino acids in its mature state. MMP2 is mainly expressed in placenta, lung, pancreas, ovary, spleen, and intestine. MMP2 is secreted as a pro-enzyme (pro-MMP2) and is activated by proteolytic cleavage by other MMPs. The family of tissue inhibitor metalloproteinases (TIMPs) controls the balance between pro and active forms of MMPs. The association of MMP2 with integrin alpha-v-beta3 is important for mesenchymal cell invasion and angiogenesis. MMP2 is over-expressed in inflammatory bowel diseases. Mutations in this gene have been associated with Torg-Winchester syndrome, also called Multicentric Osteolysis Nodulosis and Arthropathy (MONA).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.