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In the mouse, Interferon-gamma (IFN-γ) is a homodimeric glycoprotein of 133 amino acids. Interferons (IFNs) were discovered due to their antiviral effects. In humans, there are three families of IFNs: IFN type I (IFN-α, ß, ω, ε, and κ), IFN type II (one single representative, IFN-γ), and IFN type III (IFN-λ1-3). Antigens and mitogens stimulate the production of IFN-γ in Natural Killer and activated helper T lymphocytes. IFN-γ shows multiple effects. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens and leukocyte adhesion molecules. It also activates macrophages and enhances the secretion of immunoglobulins by B cells. Response to IFN-γ is mediated by the heterodimeric IFN-γ receptor. Importantly, IFNs have proven to be effective in the treatment of several viral infections and cancers.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.