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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
MCP2 is Monocyte Chemoattractant Protein 2, also known as HC14, CCL8, SCYA10 and SCYA8. It belongs to the monocyte chemoattractant protein (MCP) family. Studies show that unlike all other MCP chemokines, mouse MCP2 is an agonist for the chemokine receptor CCR8 but not for CCR2. Mouse MCP2 induces migration and calcium flux in TH2-R2A cells. Mouse MCP2 is constitutively expressed in the skin and is abundant in skin lymph nodes.
|Assay Target||MCP2, CCL8|
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|