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In the mouse, Interleukin 10 (IL10), first characterized as a Cytokine Synthesis Inhibitory Factor (CSIF), is a 160 amino acid protein noncovalently associated in a homodimer. In humans, IL10 is produced by various T cell populations, monocytes, macrophages, and other cell types. IL10 activity is mediated by the heteromeric IL10 receptor (IL10R). IL10 inhibits interferon-? synthesis in Th1 cells and monocytes, and inhibits IL12 production by monocytes. It also impairs the proliferation and cytokine synthesis of CD4+ T cells, without having a direct inhibitory affect on CD8+ T cells. On the other hand, IL10 has a stimulatory influence on B cells. In general, its main biological functions are to limit and terminate the inflammatory responses, block proinflammatory cytokine secretion, and regulate the differentiation and proliferation of several immune cells.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.