The AlphaLISA® Human Leptin Detection Kit is designed for detection and quantitation of human leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Due to the presence of leptin binding proteins in serum or other matrices, an acid-dissociation step might be required before performing the AlphaLISA assay.
true falseFor research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Leptin is a 16-kDa hormone secreted by differentiated adipocytes. It regulates energy homeostasis as a result of its action on the brain via hypothalamic neuronal pathways expressing the leptin receptor (LR). Hereditary deficiency of leptin, or functional LRs, causes severe obesity in humans and mice. Leptin-mediated signaling has been implicated in the regulation of food intake, energy expenditure, lipid and carbohydrate metabolism, and reproductive, neuroendocrine, thyroid, and immune function.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
| Assay Target | Leptin |
|---|---|
| Assay Target Class | Hormone |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Metabolic |
| Unit Size | 5,000 assay points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.
AlphaPlate-1536, Light gray, untreated, Case of 50
List Price : USD1242.0
AlphaPlate-384, Light gray, untreated, Case of 50
List Price : USD546.0
OptiPlate-384, White Opaque 384-well Microplate, Case 50
List Price : USD295.0
