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KRAS, a member of the Rat sarcoma (RAS) oncogene family, is implicated in various biological processes, such as cell proliferation, cell survival, and cell metabolism. This gene is well known to be mutated in many cancer subtypes, inducing uncontrolled proliferation and cell metabolism modifications, and thereby contributes to the Warburg effect in cancer cells. SOS1 (Son of sevenless homolog1) is a guanine nucleotide exchange factor (GEF) which interacts with KRAS to turn GDP into GTP and activates KRAS. There is a positive feedback loop between SOS1 and RAS that increases RAS signaling. The upregulation of the KRAS/SOS1 interaction leads to cancer phenotypes. WT/SOS1 identification of inhibitors or identification of GTP competitors are the two major strategies to control biological processes involved in cancer growth, by reducing the KRAS activity as well as the associated pathways. Utilize the KRAS WT/SOS1 binding assay kit to further your KRAS drug discovery strategy.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been ...
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.