KIM-1 (human) AlphaLISA Detection Kit, 5,000 assay points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Kidney Injury Molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor1 (HAVcr-1) and T-cell immunoglobulin and mucin domain 1 (TIM-1), is a type 1 transmembrane protein containing mucin and immunoglobulin domains. KIM-1 is widely expressed with the highest levels in kidney and testis. KIM-1 serves as a biomarker of renal damage, specifically proximal tubular injury. Patients with renal carcinoma or acute tubular necrosis, for example, experience a drastic increase of KIM-1 in their serum or urine. This detection kit is designed to quantify KIM-1 in urine, serum and cell culture media.