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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Influenza A is one of 3 types of influenza viruses (A, B, and C). This virus is composed of two main types of glycoproteins that are located on the surface of the viral envelope. Influenza A virus has one of sixteen possible Hemagglutinin surface proteins and one of nine possible Neuraminidase surface proteins which ultimately causes influenza in birds and some mammals. The primary function of a nucleoprotein (NP) is to encapsidate the negative strand viral RNA for the purpose of RNA replication, transcription and packaging.
|Assay Target||Influenza A NP|
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Drug Repurposing can be an effective way to identify treatments for diseases, especially when time is of the essence.
Research scientists at National Center for Advancing Translational Sciences (NCATS), a part of the NIH, embarked on a drug repurposing strategy as the quickest route to gene ...