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IL23A, also known as human interleukin 23, is a pro-inflammatory cytokine produced by T-helper cells. Interleukin-23 is a heterodimeric cytokine composed of an IL-12p40 subunit that is shared with IL-12 and the IL-23p19 subunit. It plays major role in inflammation in disorders such as arthritis and psoriasis, and has been demonstrated as being the primary generator of inflammation in multiple sclerosis, a role previously thought to be mediated by the related cytokine interleukin 12. The present kit is designed to detect IL23A in serum, plasma, cell culture supernatants, and cell lysates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
This manual explains how to run the AlphaLISA no-wash human IL-23 detection assay.