For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Interleukin 5 (IL-5) is a pleiotropic cytokine produced primarily by Th2 cells, mast cells, and eosinophils. It is synthesized as a precursor maturing into an active protein which is heavily glycosylated and covalently linked in a homodimer by a disulfide bond. IL5 supports the proliferation and differentiation of mouse B cells, and enhances IgM, IgG1, IgA, IgE secretion. Its key function is to mediate activation, maturation and survival of eosinophils and is strongly implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.
Assay Target | IL5 |
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Assay Target Class | Cytokine |
Automation Compatible | Yes |
Detection Method | Alpha |
Format | Microplates |
Product Brand Name | AlphaLISA |
Shipping Condition | Blue Ice |
Therapeutic Area | Inflammation |
Unit Size | 500 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
This manual explains how to run the AlphaLISA no-wash mouse IL-5 detection assay.