The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Interleukin 25 (IL-25), also known as interleukin 17E is a member of the IL-17 family that is secreted by lymphocytes to stimulate the production of IL-4, IL-5 and IL-13, all mediators of inflammation. IL-25 is mainly expressed by cells in the digestive system and plays a major role in immunity of the gastro-intestinal track. However, it is also involved in inflammation of those tissues. IL-25 is also known as an anti-tumor agent in these tissues, preventing colon, pancreatic and liver cancer. This kit allows for the detection of IL-25 in biological samples.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual describes how to run an AlphaLISA human IL-25 detection assay.