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IL-25 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

This product replaces AL3048.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
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AL3135C
500 assay points
2144.00 USD
 
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AL3135F
5,000 assay points
17200.00 USD
 
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AL3135HV
100 assay points
715.00 USD
 
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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 25 (IL-25), also known as interleukin 17E is a member of the IL-17 family that is secreted by lymphocytes to stimulate the production of IL-4, IL-5 and IL-13, all mediators of inflammation. IL-25 is mainly expressed by cells in the digestive system and plays a major role in immunity of the gastro-intestinal track. However, it is also involved in inflammation of those tissues. IL-25 is also known as an anti-tumor agent in these tissues, preventing colon, pancreatic and liver cancer. This kit allows for the detection of IL-25 in biological samples.

AlphaLISA® technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IL-25
Assay Target Class Cytokine
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation, Auto-Immunity
Unit Size 100 assay points
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Application Note

Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

Alpha technology offers a simple, straight forward workflow. No wash needed!

  1. Add sample
  2. Add Acceptor bead mix; incubate 1 hr
  3. Add Donor beads; incubate 30 mins
  4. Read on Alpha-enable microplate reader

Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

PDF 1 MB

Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB
Drug Discovery Screening Solutions Brochure

Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.

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