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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Interleukin 21 (IL21) is a 15 kDa cytokine that is produced by activated T follicular helper cells and natural killer T cells. It has regulatory effects on cells of the immune system such as cytoxic T cells, which destroy infected or damaged cancerous cells. In addition, IL21 is known to suppress cutaneous hypersensitivity reactions by limiting allergen specific IgE production and mast cell degranulation. High levels of IL21 are related to decreasing allergic responses, as well as controlling persistent viral infections, metastatic melanoma, and renal cell carcinoma. This kit is designated to detect and quantify the levels of IL21 in cell culture media and other biological matrices.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This manual explains how to run the AlphaLISA no-wash human IL-21 detection assay.