IgM (human) AlphaLISA Detection Kit, 5,000 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Immunoglobulin M (IgM) is the largest antibody produced by B cells. It is mainly produced in a pentameric form weighing around 900 kDa, but also in a hexameric form. Its multimeric nature gives IgM high avidity. It is mostly found in the serum, yet it is also present in secretions. IgM is a very efficient activator of the complement and appears very early during the infection process. Moreover, anti-A and anti-B antibodies responsible for blood type incompatibility are IgM molecules. Selective IgM deficiency (SIgMD) is a very rare immune disorder that results in absence or deficiency of IgM, with normal levels of other immunoglobulins, namely IgA and IgG. Hyper-IgM syndrome (HIGM) is characterized by a decrease or absence of IgG and IgA, whereas the IgM level is normal or increased.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.