The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Porcine IFNγ, the only member of the type II interferon class, is a 20 -25KD homodimer glycosylated cytokine that is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells. It is critical for innate and adaptive immunity against viral, bacterial and protozoan infections, and is involved in tumor growth. In pigs, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Porcine IFNγ has been identified as a biomarker for vaccine responses, viral, and bacterial infection. This kit is designed to detect pIFNγ in serum, plasma, cell culture supernatants, and cell lysates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...