The AlphaLISA® Human Interferon β (IFN-β) Detection Kit is designed for detection and quantitation of human IFN-β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-β 1a subtype.
This product replaces AL265.
For research use only. Not for use in diagnostic procedures.
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Mature Interferon-beta (IFN-β) contains 166 amino acids with a molecular weight of 22 kDa and is produced in large quantities by fibroblasts in response to pathogens. IFN-β is a cytokine involved in the regulation of unspecific humoral immune responses as well as in immune responses against viral infections. Only small amounts of IFN-&beta are produced under healthy conditions. However, levels can dramatically increase during viral infection, inflammation, and autoimmunity. IFN-β exhibits several biological effects including antiviral and antiproliferative activities. IFN-β has been used, with some success, as a therapeutic agent in the treatment of multiple sclerosis, as well as in chronic active hepatitis B where it appears to be most promising if the disease has not lasted longer than 5 years.
AlphaLISA® technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Oncology, Auto-Immunity|
|Unit Size||500 assay points|
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.
Alpha technology offers a simple, straight forward workflow. No wash needed!
Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
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