The AlphaLISA® immunoassay kit for human perlecan enables the detection and quantitation of human perlecan (endorepellin) in human serum and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay showed no cross reacitivity to vimentin, fibronectin, nidogen-1, laminin, or collagen I.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Perlecan is a large (4391 AA) multidomain protein, one of them being Endorepellin (3687 – 4391 AA), synthesized primarily by vascular endothelial cells and deposited in the vascular extracellular matrix. Its function is to crosslink a variety of matrix components to maintain the endothelial barrier function. Further, it can stimulate growth and regeneration of the endothelium via the modulation of certain growth factor activities. Perlecan has garnered significant interest due to its ability to suppress tumor growth upon its own suppression. Also, its levels have been observed to decrease in other diseases like atherosclerosis and diabetes. This AlphaLISA kit has been designed to detect and quantify perlecan/endorepellin in human serum and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.