PerkinElmer
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AlphaLISA High Performance Human TNFα Detection Kit, 100 Assay Points

The AlphaLISA® High Performance (HP) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL208.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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AL3157C
500 Assay Points
2016.00 USD
 
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AL3157F
5,000 Assay Points
13300.00 USD
 
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AL3157HV
100 Assay Points
800.00 USD
 
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Overview

Tumor necrosis factor alpha (TNFα) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble homotrimeric complex. TNFα plays a role in lipid metabolism, coagulation, and endothelial function and has been associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury and heart failure, and insulin resistance.

Formats

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Points (200-50,000) 100 Assay Points
Assay Target TNFα
Assay Target Class Cytokine
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Autoimmunity, Neuroscience, Cardiovascular, Oncology, Inflammation, Immuno-oncology
Unit Size 100 Assay Points
Resources, Events & More
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

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Application Note

Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

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Homogeneous Detection of Cytokines from PBMC Supernatants using AlphaLISA High Performance Assays

Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as well as the pathology of many diseases including cancer, autoimmune conditions, neurodegenerative disorders, and atherosclerosis. As such, cytokines are often used eithe ...

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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
Detecting Cytokines with AlphaLISA Guide

Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run cytokine assays, it is key to consider a multitude of factors that impact the assay performance, such as sample dilutions and cell culture format.

Utilize this guide ...

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Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

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Flyer

No-Wash Cytokine Assay Solutions

Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on other cells. Identification of cytokines, their receptors and signaling pathways, and application of these novel observations to the clinic has made the development of ne ...

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Poster

All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures

Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...

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AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

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