The AlphaLISA® High Performance (HP) Human IL-1β Detection Kit is designed for the quantitative determination of human IL-1β in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL220.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Interleukin 1 beta (IL-1β) is a central player of the immune response, involved in inflammation at local and systemic levels. IL-1β is synthesized as a 31 kDa precursor that is cleaved by caspase-1 (ICE) into the active 17 kDa form and eventually released into extracellular space. Its production has been reported in many cell types including brain and monocytic and peripheral blood mononuclear cells. After binding to its receptor, IL-1RI, IL-1β triggers a cascade of kinase signaling pathways that lead to the activation of transcription factors like NFκB and AP-1, eventually activating the expression of genes such as MIP-2 and C-reactive protein.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Points (200-50,000)||5000 Assay Points|
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Autoimmunity, Neuroscience, Oncology, Inflammation, Metabolic, Immuno-oncology|
|Unit Size||5,000 Assay Points|
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