The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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NGAL/lipocalin-2 is a 25 kDa protein that is generated by inflammatory cells when encountering bacteria. Binding of bacteria to toll-like receptors generate the secretion of NGAL, which binds to bacterial iron-binding proteins and deactivates them. It is also secreted upon stimulation of immune cells by inflammatory cytokines such as TNF. NGAL shows elevated levels in many pathologies related to inflammation such as inflammation, pneumonia, kidney failure, obesity, cancer, meningitis, bladder infection, Alzeimer’s disease and others. As such, it is a valuable marker in all sort of pathologies in medias such as serum, plasma, urine and even saliva. It is also secreted in large amounts by some cancer cells.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.