The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
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NGAL/lipocalin-2 is a 25 kDa protein that is generated by inflammatory cells when encountering bacteria. Binding of bacteria to toll-like receptors generate the secretion of NGAL, which binds to bacterial iron-binding proteins and deactivates them. It is also secreted upon stimulation of immune cells by inflammatory cytokines such as TNF. NGAL shows elevated levels in many pathologies related to inflammation such as inflammation, pneumonia, kidney failure, obesity, cancer, meningitis, bladder infection, Alzeimer’s disease and others. As such, it is a valuable marker in all sort of pathologies in medias such as serum, plasma, urine and even saliva. It is also secreted in large amounts by some cancer cells.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|