For research use only. Not for use in diagnostic procedures.
Formats:
Features:
Matrix metalloproteinase 12 (MMP12, macrophage elastase ), a member of the matrix metalloproteinase family, is an enzyme composed of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. MMP12 can degrade soluble and insoluble elastin and type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. Like other MMPs, MMP12 is involved in the degradation of basement membranes and thus cancer development. This kit has been designed for the detection of Human MMP12 in serum and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Assay Target | MMP-12 |
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Assay Target Class | Protein |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA |
Shipping Condition | Blue Ice |
Therapeutic Area | Cancer |
Unit Size | 5,000 assay points |