For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Features:
IL17F, also known as human interleukin 17, is a pro-inflammatory cytokine produced by T-helper cells. This cytokine has been found to inhibit the angiogenesis and endothelial cells and induce endothelial cells to produce other cytokines. Levels of this cytokine play an important role in asthma and chronic inflammatory disease such as psoriasis, multiple sclerosis, and rheumatoid arthritis. The present kit is designed to detect IL17F in serum, plasma, cell culture supernatants, and cell lysates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Assay Target | IL17F |
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Assay Target Class | Cytokine |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA |
Shipping Condition | Blue Ice |
Therapeutic Area | Inflammation |
Unit Size | 500 assay points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...