The AlphaLISA® immunoassay kit for detection and quantitation of human IgG Fc in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fcγ region of human IgG.
For research use only. Not for use in diagnostic procedures.
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Human Immunoglobulin G (hIgG) is one of the antibodies that is produced by plasma B-cells and protects the body from infections and toxifications such as viruses, bacteria, fungi and toxins. Human IgG is divided into 4 subtypes: IgG1, IgG2, IgG3, and IgG4. The molecular structure of human IgG is well known as a Y-like shape that contains Fab and Fc regions. The Fc portion of IgG is widely used in Fc fusion protein therapeutics for protein or antibody drug discovery and development. There are more than a dozen Fc-fusion protein drugs are available on the market to treat various human diseases. The Human IgG Fc AlphaLISA Detection Kit specifically detects human IgG Fc portion of all IgG subtypes (IgG1, IgG2, IgG3, and IgG4).
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target||IgG, Fc|
|Assay Target Class||Antibody|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA TRF|
|Shipping Condition||Blue Ice|
|Unit Size||5000 data points|
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.
Alpha technology offers a simple, straight forward workflow. No wash needed!
Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.
This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.