PerkinElmer
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IgG Fc (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for detection and quantitation of human IgG Fc in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fcγ region of human IgG.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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AL3129C
500 data points
1605.00 USD
 
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AL3129F
5000 data points
10600.00 USD
 
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AL3129HV
100 data points
1313.00 USD
 
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well, using a 100 μL reaction volume (10 μL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Human Immunoglobulin G (hIgG) is one of the antibodies that is produced by plasma B-cells and protects the body from infections and toxifications such as viruses, bacteria, fungi and toxins. Human IgG is divided into 4 subtypes: IgG1, IgG2, IgG3, and IgG4. The molecular structure of human IgG is well known as a Y-like shape that contains Fab and Fc regions. The Fc portion of IgG is widely used in Fc fusion protein therapeutics for protein or antibody drug discovery and development. There are more than a dozen Fc-fusion protein drugs are available on the market to treat various human diseases. The Human IgG Fc AlphaLISA Detection Kit specifically detects human IgG Fc portion of all IgG subtypes (IgG1, IgG2, IgG3, and IgG4).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IgG, Fc
Assay Target Class Antibody
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name DELFIA TRF
Shipping Condition Blue Ice
Unit Size 5000 data points
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Application Note

Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

Alpha technology offers a simple, straight forward workflow. No wash needed!

  1. Add sample
  2. Add Acceptor bead mix; incubate 1 hr
  3. Add Donor beads; incubate 30 mins
  4. Read on Alpha-enable microplate reader

Download this application note to see how no-wash AlphaLISA® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

PDF 1 MB

Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

Guide

ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB