The AlphaLISA® Human Hyaluronic Acid Detection Kit is designed for detection and quantitation of human hyaluronic acid in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay. The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Hyaluronic Acid (HA), also known as hyaluronan or hyaluronate, is a large molecule (MV> 500KD) and is distributed throughout the body connective tissues. HA is an important component of synovial fluid, articular cartilage, skin, and extracellular matrix and play critical role in joints, cartilage, and skin health and the integrity of cells and cell functions. It has been extensively used in skin care products (cosmetic are) and in un-healthy joints treatments. It has been reported that the patients with fibrosis has significant increases in HA in serum and plasma. The present kit is designed to detect hHA in serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In this AlphaLISA assay, a biotinylated human aggrecan binds to the Streptavidin-coated Donor beads and human aggrecan is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target||Hyaluronic Acid|
|Assay Target Class||Aminoglycan|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.