Fibrinogen (human) AlphaLISA Detection Kit, 100 assay points

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Plasma fibrinogen is a large hexamer glycoprotein (Mr=340,000), containing two sets of three different chains (?, ?, and ?). Synthesized in the liver and circulating at a concentration of around 2-4 mg/ml, it plays a role in blood clotting and inflammation. During normal blood coagulation, a cascade activates the prothrombin to convert soluble fibrinogen into insoluble fibrin strands. These strands are then cross-linked by factor XIII to form a blood clot. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, displaying vasoconstrictor and chemotactic activities. Many studies have shown that elevated fibrinogen is a major risk factor for atherosclerosis and associated with an increased risk of ischemic heart disease (IHD), stroke and other thromboembolism. Low plasma fibrinogen concentrations are associated with an increased risk of bleeding due to impaired primary and secondary hemostasis.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.