Human epidermal growth factor receptor 2 (ErbB-2, NEU, or HER2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The standard consists of the extracellular domain of human HER2 fused to the Fc region of human IgG1. This standard is already provided in the AlphaLISA human ErbB2 kit, but can be ordered separately.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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This standard should be reconstituted in 100 µL Milli-Q® grade water. The reconstituted analyte should be used within 60 minutes or aliquoted into screw-capped polypropylene vials and stored at -20°C for further experiments. Avoid multiple freeze-thaw cycles. One vial contains an amount of analyte sufficient for performing 10 standard curves.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||1 µg|
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.