For research use only. Not for use in diagnostic procedures.
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
For the production of biotherapeutic molecules, recombinant methods are most often used. Efficiency depends on several parameters, including the source organism used. Recently, the human cell-line HEK 293 has shown much attraction to users, since it is a human, well-characterized cell line.
One of the requirements of production of recombinant biotherapeutic proteins is the absence of proteins from the host cell others than the target after purification. This is to avoid toxicity and unwanted immune responses. In order to confirm this, an AlphaLISA kit designed to measure the amounts of host cell proteins from HEK 293 cells has been created.
|Assay Target||HEK293 HCP|
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|