PerkinElmer
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HEK293 HCP AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for HEK293 host cell proteins (HCP) enables the quantitative determination of contaminating HEK293 host cell proteins in buffer, culture media, lysis buffer, and cell lysates for bioprocess applications using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL3127HV
100 Assay Points
1259.00 USD
 
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AL3127C
500 Assay Points
1539.00 USD
 
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AL3127F
5,000 Assay Points
10200.00 USD
 
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

For the production of biotherapeutic molecules, recombinant methods are most often used. Efficiency depends on several parameters, including the source organism used. Recently, the human cell-line HEK 293 has shown much attraction to users, since it is a human, well-characterized cell line.

One of the requirements of production of recombinant biotherapeutic proteins is the absence of proteins from the host cell others than the target after purification. This is to avoid toxicity and unwanted immune responses. In order to confirm this, an AlphaLISA kit designed to measure the amounts of host cell proteins from HEK 293 cells has been created.

Specifications

Assay Target HEK293 HCP
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Biologics/Bioprocess
Unit Size 5,000 Assay Points
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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB