The AlphaLISA® immunoassay kit for human COX-2 enables the detection and quantitation of human COX-2 in cell culture supernatants and cell extracts using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human COX-1 nor with sheep COX-2.
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COX-2 is a homodimer of 70 kDa subunits that is also known as cyclooxygenase-2, prostaglandin epoxide synthase or prostaglandin G synthase. It is a heme-bearing membrane associated enzyme whose function is to attach an epoxy group to arachidonic acid, creating prostaglandins G or H. This epoxide is then used by other enzymes to generate prostaglandins with oxygen groups on their structure. COX-2 is one of the most important mediators of inflammation since the molecules it generates can activate cells to produce inflammatory cytokines. Also, neurons involved in pain detection bear receptors for these prostaglandins, making it one of the prime target for anti-pain analgesics including the popular aspirin (salycilyc acid), Tylenol™ (acetaminophen) or Advil™ and Motrin™ (ibuprophen).
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Shipping Condition||Dry Ice|
|Unit Size||5,000 assay points|