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AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit, 5,000 Assay Points

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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100 assay points
871.00 USD
500 assay points
2707.00 USD
5,000 assay points
15600.00 USD
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The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput.

  • Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns
  • Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models
  • Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6

The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


Assay Target Histone H3
Assay Target Class Histone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Acetylation
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Epigenetics
Unit Size 5,000 assay points
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Technical Note

Technical Note: AlphaLISA (H3K27ac) Cell Based Kit

The AlphaLISA technology allows performing no-wash homogeneous, proximity immunoassays using Alpha Donor and AlphaLISA, Acceptor beads. In this technical note, we present an optimized, assay for measuring changes in the levels of H3K27ac after treatment, of cells with sodium butyrate and Trichostatin A (TSA), two nonselective, histone deacetylase (HDAC) inhibitors. Following a, homogeneous histone extraction protocol, the mark of interest, is detected by the addition of a biotinylated anti-Histone H3, (C-terminus) antibody and AlphaLISA Acceptor beads conjugated, to an antibody (Ab) specific to the mark. The biotinylated antibody is, then captured by Streptavidin (SA) Donor beads, bringing the two, beads into proximity. Upon laser irradiation of the Donor beads, at 680 nm, short-lived singlet oxygen molecules produced by the, Donor beads can reach the Acceptor beads in proximity to generate, an amplified chemiluminescent signal at 615 nm.

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