The AlphaLISA® immunoassay kit for human and mouse insulin enables the quantitative determination of insulin in buffer, cell culture media, serum, cell lysates and cell supernatants using a homogeneous AlphaLISA assay (no wash steps).
This kit is the new and improved version of the AL204 and AL350 kits.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Insulin is produced in the pancreas by Beta-cells of the Langherans islets as a 110 amino acid proinsulin hormone. Proinsulin is post-translationally cleaved into two chains (peptide A of 21 amino acids and peptide B of 30 amino acids) that are covalently bonded through two disulfide bonds and released when blood glucose levels rise. After 30 minutes, the blood concentration rises from roughly 50 pmol/L to 300-400 pmol/L. Insulin regulates glucose and lipid metabolism and has been linked to several disorders, including diabetes, heart disease, and obesity.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Points (200-50,000)||100 Assay Points|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Target Species||Human, Mouse|
|Unit Size||100 Assay Points|
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