Galectin-9 (human) AlphaLISA Detection Kit, 500 assay points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Galectin-9 belongs to a large family of beta-galactoside-binding proteins. Galectin-9, also known as LGLAS9, Ecalectin and UAT, is a mammalian lectin with a molecular weight around 50 kD. Galectin-9 is expressed in a variety of cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells. Galectin-9, as an immuno oncology checkpoint biomarker, induces cellular apoptosis in part by direct binding to TIM-3. It is also involved in cell aggregation, adhesion, and chemotaxis; it also induces regulatory T cells and suppresses Th1 and Th17 responses. This kit has been designed for the detection of human Galectin-9 in serum, cell lysate, and cell culture supernatants.