For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Features:
Granulocyte colony-stimulating factor, G-CSF (also named Colony Stimulating Factor-3, CSF3), is a 20 kDa cytokine that specifically stimulates the proliferation and differentiation of the progenitor cells of granulocytes. It has been shown to reverse the neutropenia associated with cytotoxic chemotherapy in bone marrow and haemopoietic stem cell transplantation. It’s also a powerful stimulus of resistance to infections, including enhanced phagocytosis, killing of microorganisms, and the production of pharmacologically active products by mature granulocytes and macrophages.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Assay Target | G-CSF |
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Assay Target Class | Cytokine |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Format | Microplates |
Product Brand Name | AlphaLISA |
Shipping Condition | Blue Ice |
Therapeutic Area | Inflammation |
Unit Size | 500 assay points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.