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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
CYR61 is cysteine rich angiogenic inducer 61, also known as CCN1 is a 40-45 kDa signaling protein associated with the extracellular matrix. It regulates cellular proliferation, apoptosis, differentiation, migration, and adhesion through its interactions with different integrin receptors and through its heparin binding activity. Human CYR61 often promotes tumor growth via neovascularization, and most human tumor-derived cell lines express this protein. Therefore, CYR61 is considered a biomarker for tumor growth and for diseases related to chronic inflammation. This kit has been designed for the detection of human CYR61 in serum and cell culture media.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.